|Aasheim, Marnie - PLNT PATH, NDSU, FARGO ND|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2005
Publication Date: July 30, 2005
Citation: Edwards, M.C., Aasheim, M. 2005. Infectious clones of a white leaf strain of barley stripe mosaic virus. American Phytopathological Society Abstracts. Vol. 95(6):S27. Technical Abstract: Barley stripe mosaic virus (BSMV) is the rather well-characterized type member of the Hordeivirus genus. Its genome is comprised of three single-stranded RNAs, designated alpha, beta, and gamma. A “band-aid” method was developed a number of years ago for generating full-length cDNA clones of BSMV from which infectious RNA could be transcribed (Petty et. al., 1988 Gene 74:423-432; Petty et. al., 1989 Virology 171:342-349). Since then, the ability to transcribe infectious RNA from such clones has led to a variety of studies incorporating a reverse genetics approach to the investigation of viral properties including pathogenicity, virulence, movement, and seed transmission. We have now used an alternative PCR-based cloning procedure to generate infectious clones of a white leaf strain of BSMV designated CV36. Extreme bleaching of infected leaves results in a dramatic white leaf symptom in infected barley that is quite different from the typical symptoms induced by BSMV. Sequences of full-length clones for each of the genomic RNAs are being determined and the clones characterized via infectivity, recombination, and mutation analyses to identify the viral sequence responsible for this distinctive phenotype.