ARS | Publication request: MICROARRAY ANALYSIS OF TRANSGENIC WHEAT OVER-EXPRESSING A GLUTENIN SUBUNIT RELATED TO TECHNOLOGICAL PROPERTIES.

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: January 15, 2005
Citation: Scossa, F., Stamova, B., Chingcuanco, D.L., R'Ovidio, R., Anderson, O.D., Lafiandra, D., Masci, S. 2005. Microarray analysis of transgenic wheat over-expressing a glutenin subunit related to technological properties. Proceedings of the Plant & Animal Genome XIII Conference, January 15-19, 2005, San Diego, CA. p. 241.

Technical Abstract: The effects of transgene expression in plants, whether direct or indirect, are relevant to basic research questions, industrial applications, and the issue of “Substantial Equivalence” of GM plants, because transgenes can affect other components of plant metabolism. Wheat endosperm proteins are of major importance in regard to the nutritional and technological properties of wheat flours. Consequently, the potential effects of transgene introduction on these proteins deserve consideration. We focused our studies on a transgenic wheat line over-expressing a gene for a low-molecular-weight glutenin subunit (LMW-GS), which is correlated with dough quality. Preliminary proteomic data showed a high level of differential expression in some classes of wheat seed proteins. We are now carrying DNA microarray experiments to compare gene expression profiles of the wild type cultivar, the “null” genotype (derived from the transgenic line, but has lost the transgene by segregation), and the transformed line expressing the transgenic protein. Our aim is to identify the differentially expressed genes throughout the process of grain filling.