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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Characterization of a Powdery Mildew Resistance Gene in Wheat Cultivar Suwon 92

Authors
item Xu, Xiangyang - OSU
item Bai, Guihua
item Carver, Brett - OSU
item Shaner, Gregory - PURDUE UNIVERSITY
item Hunger, Robert - OSU

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 10, 2005
Publication Date: April 1, 2006
Citation: Xu, X., Bai, G., Carver, B., Shaner, G., Hunger, R. 2006. Molecular characterization of a powdery mildew resistance gene in wheat cultivar suwon 92. Phytopathology 96:496-500.

Interpretive Summary: Powdery mildew (PM) is an important leaf disease of wheat worldwide. The objectives of this study were to characterize the powdery mildew resistance gene in Suwon 92 and identify molecular markers tightly linked to the resistance gene for use in marker-assisted breeding. A population of recombinant inbred lines was derived from a cross between a PM resistant cultivar, Suwon 92, and a susceptible cultivar, CI 13227. The population were tested for adult-plant resistance to PM and characterized with two types of molecular markers and a morphological marker. The powdery mildew resistance gene in Suwon 92 was located on the short arm of chromosome 1A, which is close to the gene conferring hairy glume (Hg). Some of markers may have utility to be used in marker-assisted breeding for powdery mildew resistance in breeding programs.

Technical Abstract: Powdery mildew, caused by Blumeria graminis (DC) E.O. Speer f. sp. tritici, is an important foliar disease of wheat worldwide. Pyramiding race-specific genes into a single cultivar and combining race-specific resistance genes with durable resistance genes are the preferred strategies to improve the durability of powdery mildew resistance. The objectives of this study were to characterize the powdery mildew resistance gene in Suwon 92 and identify molecular markers tightly linked to the resistance gene for use in marker-assisted selection (MAS). A population of recombinant inbred lines (RILs) was derived by single seed descent from a cross between Suwon 92 and the susceptible cultivar, CI 13227. The RILs were screened for adult-plant infection type of powdery mildew and characterized with amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers, and a morphological marker. The powdery mildew resistance gene in Suwon 92 was located on the short arm of chromosome 1A, 8.4 to 11.6 cM away from a gene conferring hairy glume (Hg), with close linkage to eight molecular markers and one morphological marker. The linked markers explained 37.3% to 72.0% of the phenotypic variance measured in two years. Some of them may have utility to be used in MAS for powdery mildew resistance in breeding programs.

Last Modified: 7/28/2014
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