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United States Department of Agriculture

Agricultural Research Service

Research Project: CATFISH GENETICS, BREEDING, AND PHYSIOLOGY

Location: Warmwater Aquaculture Research Unit

Title: Differential Expression of Igf-I, Igf-Ii, and Toll-Like Receptors 3 and 5 Mrna During Embryogenesis in Hybrid (Channel X Blue) and Channel Catfish

Authors
item Peterson, Brian
item Bosworth, Brian
item Bourgeois, Lanie

Submitted to: Comparative Biochemistry and Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: March 14, 2005
Publication Date: May 1, 2005
Citation: Peterson, B.C., Bosworth, B.G., Bilodeau, A.L. 2005. Differential expression of IGF-I, IGF-II, and toll-like receptors 3 and 5 mRNA during embryogenesis in hybrid (channel x blue) and channel catfish. Comparative Biochemistry and Physiology 141:42-47.

Interpretive Summary: Insulin like growth factors –I and -II (IGF-I and IGF-II) play important roles in growth and development of fish. Toll-like receptors (TLRs) are molecules involved in the induction of the immune response. Evidence is growing that suggests a relationship between growth and immune function. The objective of the study was to examine changes in gene expression of IGF-I, IGF-II, TLR3, and TLR5 during development in hybrid (channel catfish x blue catfish) and channel catfish. Expression levels of TLR5 and IGF-I mRNA in channel catfish and expression levels of TLR3, IGF-I, and IGF-II mRNA in hybrids increased over time. Results of this study suggest growth (IGF-I and IGF-II) and immune (TLR3 and TLR5) associated genes could be functional and play important roles during embryogenesis and early development of hybrid and channel catfish.

Technical Abstract: Insulin like growth factors –I and -II (IGF-I and IGF-II) play important roles in growth and development of mammals. Toll-like receptors (TLRs) are pattern recognition molecules that orchestrate the induction of early innate immune response by recognition of specific sequences. Evidence is growing that suggests a relationship between growth and immune function. The objective of the study was to examine changes in gene expression of IGF-I, IGF-II, TLR3, and TLR5 during embryogenesis and early larval development in hybrid (channel catfish x blue catfish) and channel catfish. Egg samples were taken pre- and post-fertilization; embryos were collected at two stages of embryogenesis, at hatch, and at swim-up. All genes were detected in unfertilized catfish eggs. Expression levels of TLR5 and IGF-I mRNA in channel catfish and expression levels of TLR3, IGF-I, and IGF-II mRNA in hybrids increased over time (P < 0.01). Effect of time was not significant for expression of IGF-II or TLR3 mRNA in channel catfish and for TLR5 mRNA in hybrid catfish. Results of this study suggest growth (IGF-I and IGF-II) and immune (TLR3 and TLR5) associated genes could be functional and play important roles during embryogenesis and early development of hybrid and channel catfish.

Last Modified: 7/22/2014
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