|Jha, A. - PLNT SCI, NDSU, FARGO, ND|
Submitted to: In Vitro Biology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: March 30, 2005
Publication Date: July 10, 2005
Citation: Jha, A.K., Dahleen, L.S., Suttle, J.C. 2005. Effects of ethylene in barley (Hordeum vulgare L.)tissue culture regeneration. Poster P-2000. In Vitro Biology Meeting. Issue 41:35-A. Technical Abstract: Ethylene is a gaseous plant hormone that regulates numerous cellular processes from germination to flowering and senescence. It is produced under stress conditions such as tissue culture and can be physiologically significant in-vitro due to enclosed conditions. This study was conducted to determine genotype-dependent ethylene production and it’s role in regeneration of barley (Hordeum vulgare L.) callus. Six barley cultivars were examined and found to produce different amounts of ethylene during culture. The highest regeneration was observed in cultivars generating the most ethylene. The media was modified by adding the ethylene precursor, ACC (1-amino-cyclopropane-1-carboxylic acid) or the ethylene antagonist silver nitrate (AgNO3) to the media at different stages of callus culture to determine the effects of ethylene during plant regeneration. Further manipulation of ethylene synthesis and/or action will be used to identify critical times for ethylene effects on plant regeneration from recalcitrant genotypes and the results will be presented.