Author
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Evens, Terence |
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Niedz, Randall |
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Submitted to: Phycological Society of America
Publication Type: Abstract Only Publication Acceptance Date: 3/1/2005 Publication Date: 8/1/2005 Citation: Evens, T.J., Niedz, R.P. 2005. Response surface methodology for the evaluation and optimization of algal media: a foray into statistical experimental design and linear programming for the mathematically challenged. Phycological Society of America. Interpretive Summary: Technical Abstract: Studies on the mineral nutrition requirements of algae have generally been limited by our ability to manipulate ion concentrations independently of the complementary cation/anion of the salts used in media formulation. This usually results in data that is confounded, and leaves us with little room for unequivocal interpretation of results. This problem is further complicated by the myriad, complex interactions between the essential elements, which have profound implications when considering the interplay between physical chemistry and algal physiology. In order to address these issues we have developed a novel methodology that combines linear programming techniques with Response Surface Methodology, and allows us to quantify not only the physiological impacts of single ions but also the interactions between multiple ions. This approach also has the advantage of allowing us to quantify and visualize the multi-dimensional space around the optimal and sub-optimal media formulations required for algal growth. Accordingly, we can now begin to map the hyper-volume of feasible ion combinations for any alga across the full spectrum of physiological responses. The first analysis of the non-confounded nitrogen:phosphorus:potassium requirements of Chlorella vulgaris will be used to illustrate these concepts and techniques. |
