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United States Department of Agriculture

Agricultural Research Service

Title: Conserved and Divergent Transcriptional Regulation of Seed Germination, and the Regulatory Roles of Gibberellin and Abscisic Acid in Barley Germination

item An, Yong-Qiang
item Chen, Kegui - UNIV OF WISCONSIN
item Tian, Shulan - UNIV OF WISCONSIN
item Kaeppler, Shawn - UNIV OF WISCONSIN

Submitted to: Plant Biology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: May 15, 2005
Publication Date: July 15, 2005
Citation: An, Y., Lin, L., Chen, K., Tian, S., Kaeppler, S. Conserved and divergent transcriptional regulation of seed germination, and the regulatory roles of gibberellin and abscisic acid in barley germination. Plant Biology 2005 Meeting. Paper No. 33005.

Technical Abstract: Seed germination and early seedling growth is a complex biological process requiring closely coordinated expression of numerous genes and pathways. Gibberellin and abscisic acid play central roles in regulating seed germination. To gain insight into the global transcriptional control of seed germination and the regulatory roles of GA and ABA, we surveyed transcript profiles of over 22,000 barley genes over the course of barley germination and seedling growth, and in de-embryonated barley aleurone tissues treated with GA and ABA. A large number of genes showed a significant increase or decrease in accumulation of their corresponding mRNAs during seed germination and seedling growth. Consistent with the roles of ABA and GA in germination, many ABA up-regulated genes showed dramatic decrease in the steady mRNA level while expression of many GA inducible genes increased significantly during seed germination. The detailed results of the experiment will be presented. We also compared transcript profiles of barley and Arabidopsis germination, and identified a set of putative Arabidopsis and barley orthologous genes with similar expression patterns during germination. The preservation of the conserved expression patterns since the divergence of Arabidopsis and barley, suggests that the genes may be subject to functional constraints and may play a role in the shared features of their seed germination. The genes with divergent expression patterns may be partly attributed to the distinct characteristic of barley and Arabidopsis germination. We conducted transcript profiles of rice germination using Affymetrix rice GeneChip. We are integrating the data into the comparative studies to reveal the conserved and divergent expression patterns among all species, monocot and dicot plant species. The data will be available for discussion.

Last Modified: 5/5/2015
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