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United States Department of Agriculture

Agricultural Research Service

Title: A Multiplex Fluorogenic Pcr Assay for Rapid Detection and Differentiation of Pseudorabies Virus (Aujeszky's Disease Virus)

item Lager, Kelly
item Richt, Juergen
item Yoon, Kyoung-Jin - IOWA STATE UNIVERSITY

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2005
Publication Date: April 4, 2005
Citation: Ma, W., Lager, K.M., Richt, J.A., Yoon, K. 2005. A multiplex fluorogenic PCR assay for rapid detection and differentiation of pseudorabies virus (Aujeszky's disease virus). Marker Vaccines and Differential Diagnostic Tests in Disease Control and Eradication, April 4-6, 2005, Ames, Iowa. p. 24.

Technical Abstract: Pseudorabies has recently been eradicated from the U.S. domestic swine herd, but still exists in many swine producing regions throughout the world. A key factor to the successful control and eradication of pseudorabies in the U.S. has been the ability to distinguish animals naturally infected with a wild-type pseudorabies virus (PRV) from animals vaccinated with a marker vaccine. A critical need for the current PRV surveillance program in the U.S. is the rapid detection of PRV. For this reason, a multiplex real-time PCR using TaqMan chemistry was developed and evaluated for its capability in the detection and differentiation of field and vaccine strains of PRV. The PCR assay could detect all PRV isolates from diagnostic submissions and vaccine strains in a differential manner. The analytic sensitivity of the assay was approximately 0.1 PFU per reaction. The assay was specific for PRV as no positive results were obtained from testing common swine viral pathogens and other herpesviruses. These observations demonstrated the potential application of the developed multiplex real-time PCR to rapid and specific detection of PRV in domestic and feral swine.

Last Modified: 4/22/2015
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