ARS | Publication request: CHARACTERIZATION OF SEQUENCES UP-REGULATED IN PEACH BARK IN RESPONSE TO LOW TEMPERATURE

Submitted to: American Society of Horticulture Science Meeting
Publication Type: Abstract Only
Publication Acceptance Date: April 25, 2005
Publication Date: July 17, 2005
Citation: Bassett, C.L., Farrell, Jr., R.E., Artlip, T.S., Norelli, J.L., Wisniewski, M.E. 2005. Characterization of sequences up-regulated in peach bark in response to low temperature. American Society of Horticulture Science Meeting. HortScience. 40:1130.

Technical Abstract: Genes whose expression is regulated by exposure to low temperature (LT) in peach (Prunus persica L. Batsch.) bark were identified by PCR suppression subtractive hybridization. Among the genes identified by this technique were several which had previously been associated with LT responsiveness as well as a few which have not been reported to be regulated by cold. Genes represented by the first group included Ppdhnl previously characterized as a seasonally expressed gene predominantly seen in bark tissue collected in winter months. A novel dehydrin found in this study, Ppdhn3, was also observed to be up-regulated at LT and seasonally expressed. Two genes not previously associated with LT response were found to be up-regulated at 5 degrees C. These genes encode a polypeptide related to some unknown mitochondrial process (pptarlp) or a transducin-like protein (Pptlp1) which may be associated with signal transduction. Expression of these genes with respect to seasonal variation and drought stress is compared to genes from peach bark (Ppdhnl and Ppdhn2) whose patterns of expression in different seasons and under water deficit are well documented.