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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #177098
Title: FIRST REPORT OF STRAWBERRY AS A NATURAL HOST OF APPLE MOSAIC VIRUS

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/21/2005
Publication Date: 4/10/2005
Citation: Tzanetakis, I.E., Martin, R.R. 2005. First report of strawberry as a natural host of apple mosaic virus. Plant Disease. 89:431.

Interpretive Summary: Apple mosaic virus (ApMV) has been reported to naturally infect a number of hosts in the Rose family including rose, apple, raspberry as well as several hosts in other plant families such as hops and birch, but has not been reported to naturally infect strawberry, though it has been grafted into strawberry experimentally. In an attempt to develop a detection method for strawberry leafroll, we isolated virus-specific dsRNA then cloned and sequenced it. The dsRNA pattern suggested possible infection with a crinivirus and an ilarvirus. Analysis of the sequences obtained confirmed this result. However, the ilarvirus obtained was not expected since Apple mosaic virus has not been known to infect strawberry naturally. Two sets of primers were designed in an effort to develop a rapid laboratory test for this virus. With both sets of primers we were able to amplify fragments of ApMV from strawberry (ApMV-str) but not from several other hosts infected with this virus. We then looked at all the sequence information available for this virus from other hosts and designed a set of primers that should detect all strains of the virus. When we used these primers we were able to detect ApMV in strawberry and rose.

Technical Abstract: In an attempt to develop a detection method for strawberry leafroll, dsRNA was extracted and cloned, as described elsewhere, from an infected plant of Fragaria vesca 'UC-5' (CFRA 9026 maintained at the USDA-ARS National Clonal Germplasm Repository in Corvallis, OR). The dsRNA pattern suggested possible infection with a crinivirus and an ilarvirus. Analysis using BLAST to search GenBank showed that clones with sequence homology to two criniviruses; Strawberry pallidosis associated virus (SPaV) and Beet pseudo-yellows virus (BPYV), as well as ApMV were obtained from this leafroll infected plant. Sequences of amplicons of the three viruses from this plant have been deposited in GenBank with accession numbers, AY854050, AY854051 and AY854052 for ApMV, BPYV and SPaV, respectively. Two sets of primers were designed to amplify fragments of ApMV from strawberry (ApMV-str) and one set of degenerate primers to amplify ApMV from several hosts. With the ApMV-str specific primers we were able to detect the ApMV from strawberry but not from Rose, whereas with the degenerate primers we were able to detect ApMV in strawberry and rose. Amplicons obtained with all three sets of primers were sequenced and were identical to the sequence obtained during the cloning process. In addition, polyclonal antibodies were used to trap ApMV from infected strawberries onto Nunc immunoassay plates. After washing, RT reactions were carried out in these wells and 5 µl of this reaction was used in PCR. The sequence of the amplicons obtained were ApMV specific as determined by sequencing.