Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: June 18, 2006
Publication Date: June 18, 2006
Citation: Gundersen, D.E., Pedroni, M.J. 2006. Transcriptional analysis of glyptapanteles indiensis polydnavirus (gibv) -encoded genes in parasitized lymantria dispar (gypsy moth).. American Society for Virology Meeting. University Park,PA June 18,2005 ; June 22,2005
Technical Abstract:
Glyptapanteles indiensis (Braconidae, Hymenoptera) is an endoparasitoid of Lymantria dispar, gypsy moth. Expression of G. indiensis polydnavirus (GiBV)-encoded genes within the pest host results in inhibition of immune response, development, and alteration of physiology, enabling successful development of the parasitoid. Analysis of transcription and expression of the nine protein tyrosine phosphatases (PTPs) and single ankyrin repeat gene (ank) encoded on GiBV genome segment F (18.6 Kb) was conducted. PTPs have presumed function as regulators of signal transduction, while ankyrin repeat genes are hypothesized to function in inhibition of NF'B signaling. Transcription of each gene was mapped by 5'- and 3'-RACE (rapid amplification of cDNA ends) and temporal expression examined in the parasitized host. Transcription of each GiBV gene was initiated at conserved ANT site upstream of the translation start site. All genes were non-spliced, with exception of PTP9 for which transcription was linked to the ank gene. All were expressed early and sustained at least 7 days post parasitization, suggesting importance in early virus-induced immunosuppression and alteration of physiology.
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