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United States Department of Agriculture

Agricultural Research Service

Title: A Method to Survey for Citrus Pathogens When Biological Indicator Plants Are Not Available

Authors
item Palmieri, M - UNV. DEL VALLE, GUATEMALA
item Donis, I - UNV. DEL VALLE, GUATEMALA
item Salazar, A - UNV. DEL VALLE, GUATEMALA
item Cruz, N - UNV. DEL VALLE, GUATEMALA
item Paniagua, A - UNV. DEL VALLE, GUATEMALA
item Brlansky, A - UNV. OF FLORIDA, CREC
item Guerra-Moreno, A - UNV OF FL, PLANT PATH.
item Manjunath, K - UNV. OF FL, PLANT PATH.
item Lee, Richard

Submitted to: International Organization of Citrus Virologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 7, 2004
Publication Date: November 30, 2004
Citation: Palmieri, M., Donis, I., Salazar, A.L., Cruz, N., Paniagua, A., Brlansky, A., Guerra-Moreno, A.S., Manjunath, K.L., Lee, R.F. 2004. A method to survey for citrus pathogens when biological indicator plants are not available. International Organization of Citrus Virologists Abstracts, Page 105.

Technical Abstract: Technical Abstract: The use of biological indicator plants for indexing for graft transmissible pathogens of citrus is absolutely essential. However in Guatemala there was an urgent need to conduct a survey to identify what citrus pathogens were present, and no indicator plants were available. We describe a protocol based on total nucleic acid extractions made from field trees which was used to test 86 samples collected from Guatemala for the presence of tristeza, psorosis, exocortis, cachexia, group III viroids, leprosis, and Xylella fastidiosa. Exocortis, cachexia, and group III viroids, tristeza virus, and leprosis were found. The RT-PCR assays for psorosis were negative although symptoms in the field were similar to those expected for psorosis. Biological tests are now being performed to verify these results.

Last Modified: 7/25/2014