Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: January 12, 2005
Publication Date: January 30, 2005
Citation: Connor, E.E., Szperka, M.E., Bannerman, D.D. 2005. Sequencing and mapping of the bovine flice-like inhibitory protein (flip) gene. Germplasm Release. Accession number AY882619.
FLICE-like inhibitory protein (FLIP) inhibits apoptosis and NF-' B activation induced by pro-inflammatory mediators in humans and mice. These processes are critical events in the pathogenesis of a variety of diseases in cattle, including mastitis. Because FLIP is known to moderate these events in other species, we mapped the bovine FLIP gene, determined the bovine FLIP mRNA sequence, and characterized FLIP expression in cultured primary bovine endothelial cells. Sequencing of bovine FLIP mRNA revealed approximately 83, 74, and 68% predicted amino acid sequence identity to its porcine, human, and murine orthologs, respectively. Bovine FLIP was mapped on chromosome 2 by radiation hybrid mapping.