|Botta, R. - UNIVERSITA DI TORINO|
|Mehlenbacher, S. - OREGON STATE UNIVERSITY|
Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 20, 2005
Publication Date: January 20, 2005
Citation: Bassil, N.V., Botta, R., Mehlenbacher, S.A. 2005. Additional microsatellite markers of the european hazelnut. Acta Horticulturae. 686:105-110. Interpretive Summary: The United States Department of Agriculture (USDA) - Agricultural Research Service (ARS) - National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon, preserves more than 740 hazelnuts, including true-to-name representatives of the common species. This study is part of an ongoing effort by the NCGR and the breeding program at Oregon State University in cooperation with the University of Torino to develop tools that can be used to identify commercial hazelnut genotypes. Of sixteen potential tools, eight were able to distinguish between 20 types of hazelnuts. These tools will verify the identity of hazelnut varieties in the NCGR collection and develop characteristic 'fingerprints' for each type. They will also track genetic patterns of traits for hazelnut breeding programs in Oregon and Italy.
Technical Abstract: Sixteen microsatellite-containing sequences were identified in enriched genomic libraries of the European hazelnut, Corylus avellana L. Of these, seven amplified multiple loci, one was difficult to score and eight amplified polymorphic single loci. Amplification and polymorphism of these eight primer pairs in 20 cultivars of C. avellana were initially determined by electrophoresis using 3% agarose gels. Forward primers were then fluorescently labeled and allele sizes determined by capillary electrophoresis. The addition of PIG-tails to five reverse primers was necessary to eliminate split peaks. Three microsatellite markers (CAC-AC115, CAC-B005 and CAC-B014) reliably distinguished among the cultivars. Microsatellite markers will be used to fingerprint the hazelnut genotypes in our collection and identify duplicate accessions.