Technical Abstract: The potential for transfer of antimicrobial resistant bacteria from animals to humans is cause for concern. Commensal bacteria such as enterococci may be reservoirs of resistance, thus it is important to characterize the strains isolated from animals and their environments. This study analyzed enterococci from three different poultry farms. Two houses on each farm were control houses and did not use any antimicrobials while two other houses on each farm used antimicrobials. Litter, chick boxliners, feed, and poultry carcasses were obtained from each house and cultured for the presence of enterococci. Enterococcus faecalis (1303/3487; 37.4%) and E. faecium (1155/3487; 33.1%) were isolated most often from all farms and houses regardless of antimicrobial treatment. Higher numbers of E. faecalis were isolated from untreated houses (728/1794; 40.6%) than from treated houses (575/1693; 33.9%). In contrast, less E. faecium were isolated from untreated houses (576/1794; 32.1%) than from treated houses (579/1693; 34.2%).