ARS | Publication request: GENOTYPING OF SELECTED RICE WILD RELATIVES (ORYZA SPP.) AND THEIR PROGENIES

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2004
Publication Date: January 15, 2005
Citation: Eizenga, G.C., Agrama, H., Jia, Y., Lee, F.N. 2005. Genotyping of selected rice wild relatives (Oryza spp.) and their progenies [abstract]. Plant and Animal Genome Conference. p. 147.

Technical Abstract: Rice wild relatives (Oryza spp.) are an important source of novel genes for rice improvement. Previous studies identified resistance to rice sheath blight (Rhizoctonia solani) and rice blast (Magnaporthe grisea) in wild Oryza spp. accessions. Also, a set of 180 SSR markers was used to genotype 550 rice (O. sativa) accessions. Approximately 110 of these SSRs were exploited to genotype 56 Oryza spp. accessions found in the US germplasm collection including O. barthii, O. glumaepatula, O. meridionalis, O. nivara and O. rufipogon. To further identify differences between the Oryza spp. accessions, polymorphic information content (PIC) values were determined. In addition, selected SSR markers associated with resistance (R)-genes were surveyed. Subsequently, 35 SSR markers were identified as a 'genotyping set' from the aforementioned markers to follow the introgression of selected O. barthii, O. nivara and O. rufipogon accessions into cultivated rice (O. sativa) through backcrossing. The O. sativa parent was either Ahrent, a US long grain cultivar, or Bengal, a US medium grain cultivar. The 35 SSR markers were scattered over the twelve chromosomes and chosen based on polymorphism between the two parents and/or proximity to known R-genes. Individual plants, selected from O. barthii, O. nivara and O. rufipogon accessions, were genotyped and the plant with the most representative genotype selected for developing rice mapping populations (Oryza spp./cultivated rice) using the advanced backcross method.