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United States Department of Agriculture

Agricultural Research Service

Title: Development and Characterization of Rice Deletion Mutants for Functional Genomics

Authors
item Jia, Yulin
item Zhou, Erxon - UA RREC
item Lin, Michael
item Rutger, J

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 15, 2004
Publication Date: November 15, 2004
Citation: Jia, Y., Zhou, E., Lin, M.J., Rutger, J.N. 2004. Development and characterization of rice deletion mutants for functional genomics [abstract]. In: Proceedings, 2nd International Rice Functional Genomics Conference, Tucson, Arizona. Abstract p. 178.

Technical Abstract: A large mutant population in single genetic background is useful for determining the functions of DNA sequences using both forward and reverse genetics. The objective of this study was to establish 45,000 independent mutant lines using a mid season US rice cultivar, Katy. Katy, released in 1989 from Arkansas, is a long grain with good yield potential and milling quality and contains effective and durable blast resistance genes. Katy was subsequently used as one of the major sources for US cultivar development programs and was chosen for US mutant population development. To date, 6,000 M1 lines induced by ethyl methane sulfonate (EMS), 14,000 M1 lines induced by fast neutrons and 25,000 M1 lines induced by gamma radiation were collected from 2001 to 2004. Preliminary analysis of M2 seedlings revealed defects in chlorophyll synthesis in approximately 4% of EMS M1 derived lines. A total of 10 blast susceptible mutants derived from fast neutrons and 7 lesion mimic mutants (1 from EMS and 6 from fast neutrons) were identified. Progress on advancing and characterization of these mutant lines will be described. All of these lines will be deposited as genetic stocks in the Genetic Stock Center of Oryzae at Dale Bumpers National Rice Research Center.

Last Modified: 7/27/2014
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