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United States Department of Agriculture

Agricultural Research Service

Title: Dietary Fatty Acid Composition, Dietary Boron, and Ovariectomy Affect Bone Strength and Microarchitecture

Authors
item Stoecker, Barbara - OKLAHOMA ST UNIV
item Nielsen, Forrest

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: December 1, 2004
Publication Date: March 4, 2005
Citation: Stoecker, B.J., Nielsen, F.H. 2005. Dietary fatty acid composition, dietary boron, and ovariectomy affect bone strength and microarchitecture [abstract]. The Federation of American Societies for Experimental Biology Journal. 19(4):A57.

Technical Abstract: An experiment was designed to compare effects on bone of dietary fat high in omega-3 or omega-6 fatty acids and of adequate or deficient boron (B). Female rats were fed diets containing 100 µg B/kg in a factorial arrangement with variables of supplemental B at 0 (B-def) or 3 (B-adeq) mg/kg and fat sources of 75 g safflower oil/kg diet or 65 g menhaden oil plus 10 g linoleic acid/kg diet. After 6 wk, six females per treatment were bred. Dams and pups continued on their respective diets through gestation, lactation and after weaning. Seven rats per treatment were ovariectomized (OVX) 14 weeks after weaning. At 4 wk after OVX, a 16 hour urine sample was collected and at 5 weeks after OVX animals were necropsied. Most aspects of vertebral microarchitecture were significantly impaired by OVX. Safflower oil compared to menhaden oil increased trabecular number and decreased trabecular separation in vertebrae. In B-def animals, safflower oil increased connectivity density. Urinary markers of bone resorption were affected by both dietary fat and boron. When the diet contained safflower oil, B-def compared with B-adeq rats had increased excretion of urinary helical peptide and deoxypyridinoline crosslinks. Based on finite element analysis, both strain and stiffness were significantly lower in OVX than in sham animals fed the menhaden diet. (Supported by USDA and Oklahoma Agricultural Experiment Station).

Last Modified: 11/23/2014
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