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Title: MONITORING THE SEASONAL INCIDENCE OF XYLELLA FASTIDIOSA IN GLASSY -WINGED SHARPSHOOTER POPULATIONS

Author
item Castle, Steven
item PRABHAKER, NILIMA - U CA, RIVERSIDE
item TOSCANO, NICK - U CA, RIVERSIDE

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: 12/12/2004
Publication Date: 12/12/2004
Citation: Castle, S.J., Prabhaker, N., Toscano, N.C. 2004. Monitoring the seasonal incidence of xylella fastidiosa in glassy-winged sharpshooter populations. CDFA Pierce's Disease Control Program Research Symposium. pp. 253-255.

Interpretive Summary: The seasonal incidence of Xylella fastidiosa in GWSS populations will be examined using a combination of analytical and experimental techniques. Collections of live GWSS adults will be made at various locations in southern California throughout the year at regular intervals. Live insects will be confined individually to grapevine plants (var. Chardonnay) to determine what proportion from the field transmit Xf. Following a 3 day inoculation access period, each test insect will be processed accordingly for detection of Xf by PCR ELISA, and/or culturing techniques. By examining sufficient numbers of insects from the field and comparing transmission test results to analytical results, the relative efficiencies of each technique at identifying infected or infectious insects will be determined. Moreover, the seasonal occurrence of infectious insects will be determined and may provide guidance for when to be most vigilant for protecting against primary spread of Xf into vineyards.

Technical Abstract: The seasonal incidence of Xylella fastidiosa in GWSS populations will be examined using a combination of analytical and experimental techniques. Collections of live GWSS adults will be made at various locations in southern California throughout the year at regular intervals. Live insects will be confined individually to grapevine plants (var. Chardonnay) to determine what proportion from the field transmit Xf. Following a 3 day inoculation access period, each test insect will be processed accordingly for detection of Xf by PCR ELISA, and/or culturing techniques. By examining sufficient numbers of insects from the field and comparing transmission test results to analytical results, the relative efficiencies of each technique at identifying infected or infectious insects will be determined. Moreover, the seasonal occurrence of infectious insects will be determined and may provide guidance for when to be most vigilant for protecting against primary spread of Xf into vineyards.