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United States Department of Agriculture

Agricultural Research Service

Title: Phospholipid Dynamics in Wheat Leaves During Cold Acclimation

Authors
item Bellinger, Brian
item Skinner, Daniel
item Halls, S - WASHINGTON STATE UNIV
item Garland-Campbell, Kimberly
item Siems, W - WASHINGTON STATE UNIV

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 30, 2004
Publication Date: November 1, 2004
Citation: Bellinger, B.S., Skinner, D.Z., Halls, S.C., Garland Campbell, K.A., Siems, W. 2004. Phospholipid dynamics in wheat leaves during cold acclimation. Agronomy Abstracts. #4690.

Technical Abstract: Electrospray ionization tandem mass spectrometry (ESI-MS) was used to profile the dynamics and inheritance of 40 phospholipids (PLs) in a diallel cross of five winter wheat cultivars during five weeks of cold acclimation. The 40 PLs included phosphatidic acids (PAs), phosphatidyl- cholines (PCs), -ethanolamines (PEs), -glycerols (PGs), -inositols (PIs), and -serines (PSs). Over the first four weeks of acclimation, the unsaturated PAs and PIs consistently increased in concentration and the less saturated forms consistently decreased, suggesting desaturase activity. Other PLs increased in some cultivars and decreased in others. The lyso forms of PA, PC, PE and PG also consistently increased. Several forms of PE, PC, PI and LPC sharply increased in concentration between four and five weeks of acclimation, while some forms of PA, LPA, LPG and PI sharply decreased. Two forms of PA and one form of PG increased over the first two weeks of cold exposure, then gradually decreased over the remaining three weeks. We conclude: 1) specific PL forms are involved in acclimation of winter wheat to cold temperatures; 2) fundamental change in the behavior of winter wheat occurs between four and five weeks of cold acclimation, and; 3) certain forms of the PLs, especially PAs, showed concentration changes suggestive of the involvement of phospholipid signaling in cold acclimation.

Last Modified: 12/28/2014