Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Stimulation of Midgut Stem Cell Proliferation and Differentiation by Insect Hormones and Peptides.

Authors
item Smagghe, Guy - GHENT UNIVERSITY
item Vanhassel, Wendy - FREE UNIV. OF BRUSSELS
item Moeremans, Clarina - FREE UNIV. OF BRUSSELS
item DE Wilde, Daan - FREE UNIV. OF BRUSSELS
item Goto, Shinatro - FREE UNIV. OF BRUSSELS
item Loeb, Marcia
item Blackburn, Michael
item Hakim, Raziel - HOWARD UNIV.

Submitted to: Annals of the New York Academy of Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 6, 2005
Publication Date: April 20, 2005
Citation: Smagghe, G., Vanhassel, W., Moeremans, C., De Wilde, D., Goto, S., Loeb, M.J., Blackburn, M.B., Hakim, R.S. 2005. Stimulation of midgut stem cell proliferation and differentiation by insect hormones and peptides.. Annals Of The New York Academy Of Sciences. 1040: 472-475

Interpretive Summary: If it were possible to interfere with the normal growth and healing mechanisms of the insect gut, biological control agents that attack the insect through the gut, such as bacteria and viruses, could be made more effective. Unfortunately, little is known about these mechanisms. In order to better understand how the insect's gut grows and heals, we applied the molting hormone of insects, which controls the skin-shedding cycles insects must go through to grow, and the biochemical precursor of the molting hormone to stem cells from the insect gut. Stem cells are undeveloped cells that multiply and then develop into fully mature cell types, allowing tissues to grow and repair themselves. Using gut stem cells from the cotton leafworm, it was discovered that the precursor of the molting hormone was more effective at stimulating the stem cells to multiply, while the molting hormone itself was more effective at stimulating the stem cells to develop into mature gut cells. In addition, an abundant blood protein of insects, arylphorin, was found to stimulate stem cell multiplication. These results provide important clues about how the insect gut grows and repairs itself, and can be used by scientists who want to develop ways of interfering with insect gut development and healing mechanisms.

Technical Abstract: Stem cells derived from midguts of the caterpillar Spodoptera littoralis can be induced to multiply and differentiate in vitro. Both ecdysone and 20-hydroxyecdysone had concentration dependent effects on the stem cells, however, ecdysone was more active in stimulation of stem cell proliferation, while 20-hydroxyecdysone was more active in stimulating stem cell differentiation into mature gut cells. Ecdysteroid receptors were found in midgut stem cell nuclei using the antibody 9B9. In addition, alpha-arylphorin and four midgut differentiation factors specifically stimulated proliferation and differentiation of stem cells, respectively.

Last Modified: 8/29/2014
Footer Content Back to Top of Page