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United States Department of Agriculture

Agricultural Research Service

Title: Detection of Clo (Cytophaga-Like Organism) Endosymbionts in Adults and Eggs of Citrus Leprosis Vectors, Brevipalpus Phoenicis and B. Obovatus

item Freitas-Astua, J. - CAPTA CITROS
item Astua-Monge, G. - CAPTA CITROS
item Carvalho, S. A. - CAPTA CITROS
item Locali, E. C. - CAPTA CITROS
item Rodrigues, V. - CAPTA CITROS
item Arrivaben, F. - CAPTA CITROS
item Hilf, Mark
item Gottwald, Timothy
item Machado, M. A. - CAPTA CITROS

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: January 31, 2005
Publication Date: March 30, 2005
Citation: Novelli, V., Freitas-Astua, J., Astua-Monge, G., Carvalho, S., Locali, E., Rodrigues, V., Arrivaben, F., Hilf, M.E., Gottwald, T.R., Machado, M. 2005. Detection of clo (cytophaga-like organism) endosymbionts in adults and eggs of citrus leprosis vectors, brevipalpus phoenicis and b. obovatus. Meeting Abstract.

Technical Abstract: Citrus leprosis virus (CiLV), transmitted by the Tenuipalpidae mite Brevipalpus sp., is responsible for millions of dollars in losses every year in Brazil, and has increasing importance in other countries in South and Central America. The understanding of the mechanisms involved in the pathosystem plant-virus-vector is essential for the development of preventive and economically viable strategies for controlling the disease. Several studies suggest a tight relationship among viruses and their vectors, as well as important roles of endosymbionts in the vector's ability to transmit the virus. Recent data showed the presence of endosymbionts belonging to the Cytophaga-like-organism (CLO) group in B. phoenicis obtained from coffee trees. However, there is very few information on the Brevipalpus-endosymbiont interaction. The objective of this study was to determine the presence of CLO endosymbionts in different species and stages of the Brevipalpus mite's life cycle. Total DNA from adults and eggs of B. phoenicis populations from citrus orchards and B. obovatus population obtained from Cestrum nocturnum was used for PCR using specific primers for a region within the 16S rDNA of the endosymbiont. The presence of the bacterium was confirmed in adults and eggs of all populations sampled, evidencing efficiency of the PCR for its detection and suggesting the occurrence of both horizontal and vertical endosymbiont transmission within the genus Brevipalpus.

Last Modified: 4/19/2015
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