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ARS Home » Southeast Area » Tifton, Georgia » Crop Genetics and Breeding Research » Research » Publications at this Location » Publication #173758
Title: SCREENING METHOD FOR DETERMINATION OF AFLATOXINS IN PEANUT AND CORN

Author
item AUGUSTO, JOAO - UNIV OF GEORGIA
item COULIBALY, MAMADOU - UNIV OF GEORGIA
item WILSON, DAVID - UNIV OF GEORGIA
item Holbrook, Carl - Corley
item Widstrom, Neil

Submitted to: Aflatoxin Elimination Workshop Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2004
Publication Date: 12/1/2004
Citation: Augusto, J., M. Coulibaly, D.M. Wilson, C.C. Holbrook , N.W. Widstrom. 2004. Screening method for determination of aflatoxins in peanut and corn. Proc. Aflatoxin Elimination Workshop p. 104.

Interpretive Summary: not required

Technical Abstract: Although different analytical methods have been recommended to determine aflatoxins in food and feeds in developed countries, most of these methods are costly and complex. Therefore, a rapid, easy and inexpensive method to screen aflatoxins in peanut and corn was developed. The method involved several steps including sample preparation and extraction with a relatively weak organic polar solvent (acetone-water 80:20, v/v), cleanup with anhydrous sodium sulfate and activated neutral alumina (800mg) packed in a polyethylene tube, elution with a strong organic polar solvent such as methanol (methanol, HPLC Grade), derivatization using a bromine solution, and quantification with a calibrated Fluorometer. Statistical analysis with SAS/STAT software showed high correlations between the developed method and the inimunoaffinity method (Afla Test P, manufactured by VICAM) in peanut and corn samples with adjusted R values of 0.96 and 0.98 respectively. The initial assessment of dollar cost for each column packed with activated alumina neutral is less than two dollars allowing a substantial cost saving.