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United States Department of Agriculture

Agricultural Research Service

Title: Multiresidue Analysis of Seven Anticoagulant Rodenticides by Lc/es/ms/ms

Authors
item Marek, Le Etta - MN DEPT OF AGRIC
item KOSKINEN, WILLIAM

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 12, 2005
Publication Date: March 13, 2005
Citation: Marek, L.J., Koskinen, W.C. 2005. Multiresidue analysis of seven anticoagulant rodenticides by LC/ES/MS/MS [abstract]. American Chemical Society Abstracts. p. 72.

Technical Abstract: Mice and rat populations are commonly controlled by two classes of rodenticide anticoagulants, coumarins and indandiones. However, poisoning of nontarget animals also often occurs. For cases such as these, a rapid, multiresidue method, which provides positive confirmation for both classes of anticoagulant rodenticides, is needed by diagnostic laboratories. A method was developed for the determination of seven anticoagulent rodenticides, coumafuryl, pindone,warfarin, diphacinone, chlorophacinone, bromadiolone, and brodifacoum by high performance liquid chromatography/electrospray/mass spectrometry (LC/ES/MS/MS). Separation was performed using a Zorbax RX-C8 (2.1 x 150 mm x 5 ìm) column. The mobile phase gradient elution was 60% 10 mM ammonium acetate: 40% methanol (v:v) from 0 to 4 min, increasing to 50%, to 70% and to 90% methanol at 9, 14, and 18 min, respectively, with a total run time of 24 min. Detection was by MS/MS with electrospray ionization in negative mode. Confirmation was by retention time, m/z of molecular ion and two parent daughter transitions. Recoveries from selected matrices ranged from 70-100%. Detection limits were as low as 1-5 ng g-1. The developed method was rapid and provided the simultaneous quantification and confirmation of the seven anticoagulant rodenticides.

Last Modified: 8/19/2014
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