ARS | Publication request: HAPTEN SYNTHESES AND ANTIBODY GENERATION FOR THE DEVELOPMENT OF A POLYBROMINATED FLAME RETARDANT ELISA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 13, 2004
Publication Date: March 13, 2005
Citation: Shelver, W.L., Keum, Y., Kim, H., Rutherford, D., Hakk, H., Bergman, A., Li, Q.X. 2005. Hapten syntheses and antibody generation for the development of a polybrominated flame retardant ELISA. [abstract] 229th American Chemical Society (ACS) National Meeting, San Diego, CA, 3/13-17/2005, Picogram No. 68, Abstract No. 210.

Technical Abstract: Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants that are increasingly an environmental concern. Several antibodies were developed for the polybrominated diphenyl ether flame retardant BDE-47, found in the highest concentration of any of the 209 possible congeners. Four haptens (I-IV) with different bromine and linker substitution patterns were synthesized and utilized to generate five polyclonal antibodies from goats and two polyclonal antibodies from rabbits. Competition was assessed using four different coating antigens for all seven antibodies. The coating antigen showed marked effects on competition, with homo ELISA being less competitive, demonstrating a "bridge" effect due to linker binding. The effect of structure on competition was evaluated by using BDE-47, BDE-99, BDE-100, BDE-153, and BDE-183. Using the "best" combination of coating antigen (hapten I-BSA) and primary antibody (rabbit sera from hapten III-KLH) a calibration curve was generated with a mid point inhibition concentration at 27.7 ng/mL (n=3).