Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: How Common Are Bacteriophage in Feces of U.S. Feedlot Cattle?

item Callaway, Todd
item Edrington, Thomas
item Genovese, Kenneth
item Keen, James
item Anderson, Robin
item Schultz, Carrie
item Poole, Toni
item Nisbet, David

Submitted to: Society for General Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: January 8, 2005
Publication Date: April 4, 2005
Citation: Callaway, T.R., Edrington, T.S., Genovese, K.J., Keen, J.E., Anderson, R.C., Brabban, A.D., Kutter, E., Schultz, C.L., Poole, T.L., Nisbet, D.J. 2005. How common are bacteriophage in feces of U.S. feedlot cattle [abstract]? Society for General Microbiology. p. 61.

Technical Abstract: Escherichia coli O157:H7 is a food-borne pathogen of critical importance that often colonizes cattle. E. coli O157:H7 can be specifically killed by bacteriophage (bacterial viruses); and bacteriophage treatment has been suggested as a pre-harvest intervention strategy to reduce food-borne pathogens in cattle. No systematic approach to determine the incidence of anti-E. coli O157:H7 phage (AO157P) has been previously performed. Therefore the current study was designed to determine 1) the incidence of E. coli O157:H7 and Salmonella and 2) the incidence of AO157P in the feces of feedlot steers in commercial feedlots in the United States. Fecal samples (n = 60) were collected from four feedlots in two Southern Great Plains states (total n = 240 fecal samples). Salmonella and E. coli O157:H7 were found in 3.8% and 11.7% of the fecal samples, respectively. AO157P were found in 15% of the fecal samples. AO157P were present in all four feedlots and were found in 55% of the cattle pens. Our results indicate that anti-E. coli O157:H7 is very widespread in feedlot cattle, indicating that further research into the ecological role of bacteriophage in the gastrointestinal tract is needed.

Last Modified: 4/18/2015