Location: Crop Bioprotection Research
Title: Transcriptome Dynamics of Ethanologenic Yeast in Response to 5-Hydroxymethylfurfural Stress Related to Biomass Conversion to Ethanol Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 18, 2005
Publication Date: March 15, 2005
Citation: Liu, Z., Slininger, P.J. 2005. Transcriptome dynamics of ethanologenic yeast in response to 5-hydroxymethylfurfural stress related to biomass conversion to ethanol [abstract]. Paper No. 177C. Technical Abstract: Among inhibitive compounds generated by dilute acid hydrolysis of lignocellulosic biomass, 5-hydroxymethylfurfural (HMF) is one of the major potent inhibitors for microbial fermentation. In an effort to understand HMF stress tolerance mechanisms for improved microbial performance and ethanol conversion, we investigated global transcriptome profiling of ethanologenic yeast in response to HMF stress. Under the challenge of HMF, many genes were differentially expressed significantly at varied mRNA abundance levels compared with that of a control. Significant induction and repression of gene expression in a wide range of functional categories indicated a global adaptation process in yeast at least 10 minutes after cells were exposed to HMF. At a tolerable dose of HMF, numerous genes showed a dynamic progress of expression over time. At the end of the fermentation, HMF-adapted yeast produced a comparable and even higher yield of ethanol compared with a non-HMF treated control under laboratory conditions. This presentation will cover our improved technology using 70-mer DNA oligo microarray with stringent quality control measurements developed in this study. We will demonstrate dynamic expression patterns for genes in different functional categories and report results of HMF conversion, and ethanol production of ethanologenic yeast in response to HMF stress.