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United States Department of Agriculture

Agricultural Research Service

Title: Comparison of Microbial Identification Systems, Fatty Acid Analysis and Substrate Utilization, to Identify Bacteria Associated with Poultry Processing

Authors
item Sanders, Sheriase - UGA
item Hinton, Jr, Arthur
item Frank, J - UGA
item Arnold, Judy

Submitted to: Journal of Food Protection
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: August 31, 2007
Publication Date: N/A

Interpretive Summary: Bacteria were collected from poultry products and processing areas that included broiler carcasses, cut-up chicken, and machine surfaces. The names of the species were determined by two scientific equipment systems, called MIDI and BIOLOG. The MIDI identified bacteria by the fatty acids that they produced, and the BIOLOG identified bacteria by the nutrients that they used. Twenty-four per cent of the samples received the same identification on both systems. BIOLOG identified 60% of the samples, including 90% of the gram-positive bacteria and 53% gram-negative. MIDI identified 72% of the samples, and 28% of the samples required further confirmation. Each system displayed advantages and disadvantages for bacterial identification.

Technical Abstract: Bacteria from food processing areas were identified by cellular fatty acid profiles (MIDI Sherlock) and substrate utilization profiles (BIOLOG). Sources for bacteria were the American Tissue Culture Collection, broiler carcasses, cut-up chicken, and machine surfaces. Twenty-four percent samples received the same identification on both systems. Biolog identified sixty percent of the samples, including gram-positive and gram-negative bacteria, ninety percent of the gram-positive bacteria and fifty-three percent gram-negative. MIDI identified seventy-two percent of the samples, twenty-four percent were tentative enterics and required further confirmation. Each system displayed advantages and disadvantages.

Last Modified: 12/26/2014