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United States Department of Agriculture

Agricultural Research Service

Title: A Minimal Tiling Path for Maximal Genome Coverage: International Collaboration on the Global Bac Sequencing Platform

Author
item Yu, John

Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: September 10, 2004
Publication Date: October 10, 2004
Citation: Yu, J. 2004. A minimal tiling path for maximal genome coverage: International collaboration on the global BAC sequencing platform [abstract]. International Cotton Genome Initiative Workshop. p. 24.

Technical Abstract: Cotton is an allotetraploid crop with profound gene duplication and sequence complexity. Information on the structural organization of total DNA sequences in cotton is essential not only in identifying many thousands of cotton genes, but also in understanding the functional and evolutionary potential of any gene within its complex genomic neighborhood. A complete complement of genes encoded by the cotton genome will be likely achieved if the vast sequence environment and its unknown role are examined. A reasonably complete genome sequence of an inbred cotton line can serve as a standard reference for comparison with other cotton genotypes and species. By use of a cotton physical map that consists of overlapping BAC contigs, a minimal tiling path (MTP) of cotton BAC clones (whole genome or gene-rich regions) can be determined for sequencing, which reduces sequence redundancy and increases genome coverage. Moreover, such a genomic framework provides a sequencing platform for ready international collaboration where participating countries normally prohibit the transfer of research funds across their borders. An integrated genetic, physical, transcript, and sequence map of the cotton chromosomes will lay the solid foundation for many basic and applied cotton genetic studies to understand and improve the world's leading fiber crop. This strategy has been discussed for numerous crop species over the past few years and it will be elaborated for cotton in this presentation.

Last Modified: 10/1/2014
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