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United States Department of Agriculture

Agricultural Research Service

Title: Differential Expression of Cardiac Muscle Mitochondrial Matrix Proteins in Broilers from Pulmonary Hypertension Syndrome Resistant and Susceptible Line

Authors
item Cisar, Cindy
item Balog, Janice
item Anthony, Nick - UNIVERSITY OF ARKANSAS
item Donoghue, Ann

Submitted to: Poultry Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 3, 2005
Publication Date: May 1, 2005
Citation: Cisar, C.R., Balog, J.M., Anthony, N., Donoghue, A.M. 2005. Differential expression of cardiac muscle mitochondrial matrix proteins in broilers from pulmonary hypertension syndrome resistant and susceptible line. Poultry Science. 84(5):704-708.

Interpretive Summary: Ascites is a metabolic disorder of modern broilers that is distinguished by cardiopulmonary insufficiency in the face of intense oxygen demands of rapidly growing tissues. Broilers with ascites exhibit sustained elevation of pulmonary arterial pressure and right ventricular hypertrophy, the end result of which is heart failure. It has been shown that mitochondrial function is impaired in broilers with ascites. In this study, mitochondrial matrix protein levels were compared between ascites-resistant line broilers and ascites-susceptible line broilers with and without ascites using two-dimensional (2-D) gel electrophoresis. Nine of 172 detected protein spots were present at significantly different levels in the four groups and were selected for protein identification by mass spectrometry. Two of the spots were found to contain single mitochondrial matrix proteins. Both mitochondrial matrix proteins, the dihydrolipoamide succinyltransferase component of the 2-oxoglutarate dehydrogenase complex and the alpha subunit of mitochondrial trifunctional enzyme, were significantly elevated in ascites-resistant line broilers with ascites. The elevated levels of two key proteins in aerobic metabolism in ascites-resistant line broilers with ascites observed in this study suggests that the the mitochondria of broilers with this disease may respond inappropriately to hypoxia.

Technical Abstract: Ascites is a metabolic disorder of modern broilers that is distinguished by cardiopulmonary insufficiency in the face of intense oxygen demands of rapidly growing tissues. Broilers with ascites exhibit sustained elevation of pulmonary arterial pressure and right ventricular hypertrophy, the end result of which is heart failure. It has been shown that mitochondrial function is impaired in broilers with ascites. In this study, mitochondrial matrix protein levels were compared between ascites-resistant line broilers and ascites-susceptible line broilers with and without ascites using two-dimensional (2-D) gel electrophoresis. Nine of 172 detected protein spots were present at significantly different levels in the four groups and were selected for protein identification by mass spectrometry. Two of the spots were found to contain single mitochondrial matrix proteins. Both mitochondrial matrix proteins, the dihydrolipoamide succinyltransferase component of the 2-oxoglutarate dehydrogenase complex and the alpha subunit of mitochondrial trifunctional enzyme, were significantly elevated in ascites-resistant line broilers with ascites. The elevated levels of two key proteins in aerobic metabolism in ascites-resistant line broilers with ascites observed in this study suggests that the the mitochondria of broilers with this disease may respond inappropriately to hypoxia.

Last Modified: 9/2/2014
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