Technical Abstract: An 8-year-old, male domestic short-haired cat was referred to the Matthew J. Ryan Veterinary Hospital of the University of Pennsylvania with a three day history of lethargy, inappetance, hyperemic skin nodules, coughing and vomiting. Laboratory results included a non-regenerative anemia, lymphopenia, thrombocytopenia, hypoalbuminemia, hyponatremia, increased ALT, and increased AST. Cytology of the skin nodules revealed many spindle to crescent shaped protozoal organisms, with morphology consistent with Toxoplasma gondii or Neospora caninum. Gross necropsy findings, histopathology, immunohistochemistry, and transmission electron microscopy confirmed a diagnosis of a systemic protozoal infection; however, it exhibited characteristics of N. caninum and T. gondii. A diagnosis of a T. gondii-like infection was based on the internal structures and positive reaction to rabbit polyclonal antibodies to T. gondii. Reports of toxoplasmic dermatitis are rare in the cat and dog and this is the first reported diagnosis of T. gondii-like protozoa on skin aspirates.