ARS | Publication request: PRODUCTION OF ACETONE BUTANOL FROM CORN FIBER XYLAN USING CLOSTRIDIUM BEIJERINCKII P260

Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: May 5, 2005
Publication Date: May 5, 2005
Citation: Qureshi, N., Li, X., Saha, B.C., Cotta, M.A. 2005. Production of acetone butanol from corn fiber xylan using Clostridium beijerinckii P260 [abstract]. Biotechnology for Fuels and Chemicals Symposium. p. 87.

Technical Abstract: Acetone butanol (AB or solvents) was produced from corn fiber xylan (CFX) using Clostridium beijerinckii P260. In order to perform these studies, individual components of CFX (glucose, xylose, galactose, and arabinose) were fermented using 60 g/L initial sugar. In approximately 72 h of fermentation, these sugars resulted in the production of 12.4, 11.1, 10.9, and 15.2 g/L total solvents, respectively. Under the same conditions, CFX was not fermented due to lack of bacterial growth and hydrolysis of CFX. Fermentation (pH 6.1-6.3) with 60 g/L CFX and the addition of a crude hemicellulase preparation was also unsuccessful, indicating that the hydrolysis of CFX was too slow to provide sufficient sugars for growth and solvent production. Fermentation with 60 g/L CFX and 5 g/L xylose resulted in the production of 5.2 g/L total AB, suggesting that the culture was able to partially hydrolyze CFX as 5 g/L xylose cannot produce more than 1.7 g/L AB. Further fermentation with 60 g/L xylan, 5 g/L xylose plus the crude hemicellulase preparation produced 9.6 g/L total solvents. These studies suggested that CFX can be fermented to AB by C. beijerinckii when hemicellulases and low amount of sugar are provided.