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United States Department of Agriculture

Agricultural Research Service

Title: A Hard Red Spring Wheat Intervarietal Genetic Linkage Map Constructed Using Microsatellite and Target Region Amplification Polymorphism (Trap) Markers.

item Liu, Zhaohui - PLNT PATH NDSU, FARGO ND
item Rasmussen, Jack - PLNT PATH NDSU, FARGO ND

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 3, 2004
Publication Date: January 10, 2005
Citation: Liu, Z., Friesen, T.L., Rasmussen, J.B., Hu, J., Faris, J.D. 2005. A hard red spring wheat intervarietal genetic linkage map constructed using microsatellite and target region amplification polymorphism (TRAP) markers.Plant and Animal Genome Abstracts. p.351.

Technical Abstract: A wheat mapping population of recombinant Inbred (RI) lines was developed from a cross between the NDSU hard red spring wheat (HRSW) variety 'Grandin' and the Brazilian line BR34 and provided by Dr. James Anderson. A total of 698 loci, including 327 microsatellite, 368 TRAP, 2 STS, and 1 morphological marker, were mapped using 118 RI lines. A basic frame-work map comprised of 402 markers mapping at an LOD > 3.0 accounted for a total genetic distance of 3125.4 cM with an average density of one marker per 7.7 cM. The B genome was best covered, containing 234 markers accounting for 1,187.1 cM of genetic distance (5 cM per marker), followed by A genome spanning 1,139 cM in length with 103 markers (11.05 cM per marker). Similar to previously published wheat maps, the D genome was less dense with 65 markers accounting for 799.3 cM (12.30 cM per marker), and no marker mapped to chromosome 3D. We evaluated plant height and days-to-heading (DTH) in this population to test the utility of the map for detecting QTL (Quantitative Trait Loci). Major QTL were detected on chromosomes 4BL for plant height and 5AL for DTH and explained 52 and 38 percent of the phenotypic variation, respectively. This population segregates for quality traits and resistance to multiple diseases, including tan spot, Stagonospora nodorum blotch, powdery mildew, and Fusarium head blight. Therefore, this genetic map should prove useful for the identification of markers associated with agronomically important genes and QTL.

Last Modified: 4/22/2015
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