|Lu, Huangjun - PLNT SCI, NDSU, FARGO ND|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 3, 2004
Publication Date: January 12, 2005
Citation: Lu, H., Faris, J.D. Comparative analyses of a gene rich region of wheat chromosome 5b with rice using expressed sequences.. Plant and Animal Genome XIII Abstracts p. 259. Technical Abstract: EST and genome sequencing data have provided the basis for comparative genomics between wheat and rice. In this study, markers representing expressed sequences within the wheat deletion interval 5BL 0.75-0.76 were used to determine the level of colinearity of this genomic region with rice. A population consisting of 117 recombinant substitution lines (RSLs) derived from a cross of Chinese Spring (CS) x CS Triticum dicoccoides chromosome 5B substitution line (CS-Dic5B) was used to develop a genetic map corresponding to the deletion interval. We mapped 35 expressed sequence markers as RFLPs or SSCPs, which resulted in a map of 51.4 cM in length. Of these markers, 19 (54%) detected homologous sequences in rice genome. Analyses of colinearity using expressed sequences that detected rice homologs indicated a lack of conservation. Small regions of colinearity with rice chromosomes 3 and 9 were found, but multiple breaks, interruptions, and rearrangments exist. These anomalies will make it extremely difficult to use rice as a model for positional cloning of wheat genes in this region due to the lack of conservation.