Submitted to: Midwestern Section of the American Society of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 30, 2004
Publication Date: March 21, 2005
Citation: Mousel, M.R., Nonneman, D.J., Rohrer, G.A. A refined comparative map between porcine chromosome 3 and human chromosomes 2, 7 and 16. Proc., Midwestern Section of the American Society of Animal Science. p. 11. 2005 Technical Abstract: A QTL on chromosome 3 has been identified in the MARC Meishan-White Composite resource population that is significantly correlated with ovulation rate (OR) and has a large effect (3.21 corpora lutea). The current resolution of the human-porcine comparative map of chromosome 3 does not sufficiently distinguish chromosomal breakpoints needed for the selection of positional candidate genes controlling this trait. This study placed 48 known genes, ESTs and 1 microsatellite (SB42) onto the swine chromosome 3 map by radiation hybrid (RH) mapping. The 7,000 IMpRH panel was used to map all markers and 34 markers were mapped using the 12,000 IMNpRH2 panel to clarify gene order. Data were analyzed for two-point and multipoint linkage with the IMpRH mapping tool and submitted to the IMpRH database (http://imprh.toulouse.inra.fr/). Carthagene (http://www.inra.fr/bia/T/CarthaGene/) was used to estimate multipoint marker distance and order using all public markers on chromosome 3 in the IMpRH database and those developed in this study. Five breakpoints were identified on the p arm of porcine chromosome 3, beginning at p1.7 to p1.2, that correspond to HSA 7q22.1 (98.80-101.05 Mb), 7q11.22-q11.23 (72.29-73.51 Mb), 7q11.22-q11.21 (68.50-64.86 Mb), 16p11.2 (31.40-28.41 Mb), 16p12.2-11.2 (19.42-23.75 Mb), and 16p13.13-p13.3 (11.26-2.03 Mb). The q arm had conserved synteny with HSA 2q11.2-p24.2 (96.68-18.65 Mb). There were three breakpoints in the chromosomal region that surrounded the OR QTL (p1.2-p1.4). Breakpoint 1 linked HSA 7q22.1 to 7q11.22-q11.23, breakpoint 2 did not have sufficient coverage to close the gap and breakpoint 3 connected HSA 7q11.22-q11.21 to 16p11.2. These data refine the human-porcine comparative map and placed additional genes on chromosome 3 which are within an OR QTL.