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United States Department of Agriculture

Agricultural Research Service

Title: Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase Pcr Assay for the Detection of Classical Swine Fever.

Authors
item Risatti, Guillermo
item Holinka, Lauren
item Lu, Zhiqiang
item Kutish, Gerald
item Callahan, John - TETRACORE, INC.
item Nelson, William - TETRACORE, INC.
item Tio, Brea - LAVECEN, REP. DOMINICANA
item Borca, Manuel

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: September 30, 2004
Publication Date: November 14, 2004
Citation: Risatti, G.R., Holinka, L.G., Lu, Z., Kutish, G.F., Callahan, J.D., Nelson, W.N., Tio, B.E., Borca, M.V. 2004. Diagnostic Evaluation of a Portable Real-Time Reverse Transcriptase PCR Assay for the Detection of Classical Swine Fever. Conference of Research Worksers in Animal Disese Meeting. P 194.

Technical Abstract: A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR for classical swine fever virus (CSFV) was evaluated for diagnostic sensitivity and specificity using clinical samples obtained from the Dominican Republic where the disease is enzootic. The test, using nasal swab samples taken from both symptomatic and asymptomatic animals, exceeded the diagnostic sensitivity of virus isolation (100% vs 72.4%, respectively) with little loss of specificity (98.9% vs 100%, respectively). At the herd level, 3 out of 4 infected farms were detected by virus isolation, while the CSF real time RT-PCR assay identified all four infected premises. This simple and accurate test permits rapid detection of CSFV in affected herds.

Last Modified: 9/20/2014
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