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United States Department of Agriculture

Agricultural Research Service

Title: A 19mer Peptide Insertion in the E1 Glycoprotein of Classical Swine Fever Fever Virus Affects Viral Virulence in Swine

item Risatti, Guillermo
item Kutish, Gerald
item Lu, Zhiqiang
item Holinka, Lauren
item French, Richard - UNIV. CONNECTICUT
item Sur, J - USDA, ARS, PIADC
item Rock, Daniel
item Borca, Manuel

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: September 30, 2004
Publication Date: November 14, 2004
Citation: Risatti, G.R., Kutish, G.F., Lu, Z., Holinka, L.G., French, R.A., Sur, J.H., Rock, D.L., Borca, M.V. 2004. A 19mer Peptide Insertion in the E1 Glycoprotein of Classical Swine Fever Virus Affects Viral Virulence in Swine. Conference of Research Worksers in Animal Disese Meeting. P.195

Technical Abstract: Transposon linker insertion mutagenesis of a full-length infectious clone of the pathogenic classical swine fever virus (CSFV) isolate Brescia (pBIC) was used to identify genetic determinants of CSFV virulence and host range. A virus mutant, RB-C22 (RB-C22v), possessing a 19-residue tag insertion at the carboxyl end of E1 was constructed. RB-C22v and the parental virus pBIC (pBICv) exhibited similar growth characteristics on primary porcine macrophage cell cultures although RB-C22v produced significantly smaller plaques on SK6 cell cultures. In vivo, RB-C22v was markedly attenuated in swine. In contrast with pBICv infection, where mortality was 100%, all RB-C22v- infected pigs survived infection remaining clinically normal. A delay in spread to and decreased replication of RB-C22v in the tonsils were accompanied by a delay in generalization of infection, and a 102 to 107 log10 reduction in virus titers in lymphoid tissues and blood. These results indicate that a domain of E1 glycoprotein affects swine virulence.

Last Modified: 4/21/2015
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