Page Banner

United States Department of Agriculture

Agricultural Research Service

Title: Developing Transcriptional Profiles and Gene Expression Analysis of Grape Plant Response to Xylella Fastidiosa

Authors
item Lin, Hong
item Walker, Andrew - UNIV OF CA-DAVIS

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Proceedings
Publication Acceptance Date: October 12, 2004
Publication Date: December 7, 2004
Citation: Lin, H., Walker, A. 2004. Developing transcriptional profiles and gene expression analysis of grape plant response to Xylella fastidiosa. Proceedings of CDFA Pierce's Disease Control Program Research Symposium. p. 25-27.

Interpretive Summary: The goal of this project is to characterize the molecular events in grape / Xylella fastidiosa interactions. We used highly resistant and susceptible genotypes segregated from a Vitis rupestris x V. arizonica population for this project. We developed a functional genomic approach to specifically identify PD-related gene expression profiles of susceptible and resistant responses. About 5,000 expressed genes have been sequenced from PD resistant and susceptible grape plants. These expression profiles derived from stem, leaf and shoot of resistant and susceptible genotypes throughout the course of disease development provide informative details of molecular basis of PD responses. Currently we are analyzing genes whose expressions are increased or decreased as the result of Xf infection. These research efforts will help identify genes that are expressed in the defense response and signaling recognition in PD susceptible and resistant grapes.

Technical Abstract: We developed a functional genomic approach to specifically identify PD-related transcriptional profiles in susceptible and resistant responses. Highly resistant and susceptible genotypes segregated from a Vitis rupestris x V. arizonica population were used for this study. About 5,000 expressed clones have been sequenced and annotated from forward and reverse subtractions of cDNA libraries. These expression profiles derived from stem, leaf and shoot of resistant and susceptible genotypes throughout the course of disease development provide informative details of molecular events associated with PD. We have identified 63 up/down regulated genes in response to Xylella fastidiosa infection in both genotypes. To further characterize genes involved in the host-pathogen interactions at different tissues and stages of disease development, a set of genes for microarray-based global gene expression analysis is being constructed. Currently, we are analyzing the first 20 candidate genes using Taq-Man gene expression assay method. These research efforts will help identify spatial and temporal expression genes involved in the defense response and signaling recognition in PD susceptible and resistant grapes.

Last Modified: 7/22/2014
Footer Content Back to Top of Page