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United States Department of Agriculture

Agricultural Research Service

Title: Extraction Methods for Analysis of Citrus Leaf Proteins by Two-Dimensional Gel Electrophoresis

Authors
item Zukas, Audrius
item Breksa, Andrew

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 17, 2005
Publication Date: October 27, 2004
Citation: Zukas, A.A., Breksa III, A.P. 2004. Extraction methods for analysis of citrus leaf proteins by two-dimensional gel electrophoresis. ACS Symposium, Paper #74, October 2004, Sacramento, CA.

Technical Abstract: A general procedure for two-dimensional (2-D) gel electrophoresis of Citrus leaves has been developed. We evaluated the resolution of 2-D protein patterns for Tris-HCl and KCl extractions of water soluble proteins and a phenol extraction for extraction of hydrophobic cell membrane proteins from Chandler pummelo (Citrus grandis) leaves. All three extractions provided satisfactory results. In addition, the versatility of the Tris-HCl extraction method was demonstrated by extracting leaves from six different members of the Citrus family (C, aurantium, Bouquet des fleurs (sour orange); C. tangerina cv. Dancy (mandarin); C. medica, cv. Diamante (citron); C. limon, cv. Frost eureka (lemon); C. sinensis, cv. Powell (navel orange); and C. paradisi, Redblush (grapefruit)).

Last Modified: 10/21/2014