ARS | Publication request: NEW BAC-END DERIVED MICROSATELLITE MARKERS IN COTTON (GOSSYPIUM HIRSUTUM L.) ACALA 'MAXXA'.

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 31, 2004
Publication Date: October 31, 2004
Citation: Frelichowski, J.E., Ulloa, M., Tomkins, J.P., Palmer, M., Main, D., Stelly, D., Cantrell, R.G. 2004. New Bac-end derived microsatellite markers in cotton (Gossypium hirsutum L.) Acala 'Maxxa'. ASA-CSSA-SSA Annual Meeting Abstracts. p. 5613.

Technical Abstract: A BAC library constructed from G. hirsutum 'Maxxa' was used for generation of genomic sequence information from their ends and microsatellite sequences, alternatively called simple sequence repeats (SSRs), were identified. Eight hundred sequences were selected that contained di, tri, or tetra nucleotide repeats and PCR primers were designed to flank the SSRs. The primers were assayed for length polymorphisms in PCR products with DNA of four cotton genotypes: G. hirsutum 'Maxxa', G. hirsutum TM-1, G. barbadense 3-79, and G. raimondii. Successful PCR amplification was achieved with 583 primer pairs with 289 SSR markers detecting polymorphism in presence or length of PCR products. The level of polymorphism was higher between the diploid G. raimondii and the tetraploid accessions, 'Maxxa', TM-1 and 3-79 (45%) than between the tetraploid species (23%), and within G. hirsutum the polymorphism level was 5%. Markers polymorphic between G. hirsutum and G. barbadense are being assayed on cytogenetic stocks, and 16 are currently assigned chromosomal locations. These SSR markers will be used to expand the cotton genetic map, and for the evaluation and assessment of germplasm diversity. Primer sequences of these SSRs will be made available to the cotton community.