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United States Department of Agriculture

Agricultural Research Service

Title: Molecular Cytogenetic Characterization and Seed Storage Protein Analysis of 1a/1d Translocation Lines of Durum Wheat Carrying Glu-Dld.

Authors
item Xu, Steven
item Faris, Justin
item Cai, X - PLNT SCI, NDSU, FARGO ND
item Klindworth, Daryl

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 2004
Publication Date: January 4, 2004
Citation: Xu, S.S., Faris, J.D., Cai, X., Klindworth, D.L. 2004. Molecular cytogenetic characterization and seed storage protein analysis of 1a/1d translocation lines of durum wheat carrying glu-dld.. Plant and Animal Genome Abstracts.[Online.] Available at http://www.intl-pag.org/13/abstracts/PAG13_P239.html. Plant & Animal Genomes XIII Conference, January 15-19, 2005. San Diego,CA.

Technical Abstract: The Glu-D1d allele on chromosome 1D of bread wheat encodes for high-molecular-weight (HMW) glutenin subunits (GS) 1Dx5+1Dy10, which are associated with good bread-making quality. The durum wheat lines carrying Glu-D1d were previously developed by crossing bread wheat 'Len' with durum wheat 'Langdon' and 'Renville'. In this study, two translocation lines, L252 and S99B34, were characterized using fluorescent genomic in situ hybridization (FGISH) and microsatellite markers. The FGISH patterns of the mitotic chromosomes indicated that these two durum lines have one pair of 1AS·1AL-1DL translocated chromosomes in which the terminal fragment of 1AL was replaced by a homoeologous fragment of 1DL. The 1DL fragment spans approximately one-third of the long arm of the translocated chromosome. Microsatellite marker analysis confirmed the 1AS·1AL-1DL translocation in the durum lines and determined the translocation breakpoint to be distal to Xgwm357 on 1AL. Seed storage proteins, including glutenin subunits and gliadins, were analyzed in these two 1AS·1AL-1DL translocation lines and three sib lines (L092, S99B19, and S99B33) using sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and acid (A)-PAGE. The SDS-PAGE and A-PAGE profiles demonstrated that L252 and S99B19 had the low-molecular-weight (LMW) -1 GS encoded by Glu-A3k and Glu-B3s and gliadin proteins encoded by Gli-B1 from LDN. The other three lines, L092, S99B33, and S99B34, had LMW-2 GS and gliadins encoded by Gli-B1 from Renville. Since LMW-2 GS are associated with good pasta qualities in durum wheat, the translocation lines with 1Dx5+1Dy10 and LMW-2 will be useful for developing durum cultivars with dual-purpose end-use.

Last Modified: 10/20/2014
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