Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 2004
Publication Date: January 4, 2004
Citation: Xu, S.S., Faris, J.D., Klindworth, D.L., Cai, X., Hu, J. 2004. Utilization of molecular markers in the characterization and development of germplasm of and genetic stocks in wheat.. Plant and Animal Genome Abstracts. [Online.]Available at http://www.intl-pag.org/13/abstracts/PAG13_W225.html. Plant & Animal Genomes XIII Conference, January 15-19, 2005. San Diego, CA. Technical Abstract: Modern molecular marker technologies greatly facilitate the characterization and development of new germplasm and genetic stocks in crops. During the last 30 years, Dr. L. R. Joppa developed a number of useful germplasm and genetic stocks in durum and common wheat, including various durum disomic substitutions, translocations, and synthetic hexaploid wheat lines. To provide an efficient means of maintaining these lines, we are extensively characterizing them using DNA markers and gel electrophoresis of seed storage proteins (glutenin subunits and gliadins). Thus far, three sets of Langdon durum-Triticum dicoccoides disomic substitution lines, five durum 1D/1A translocation lines, and 40 synthetic wheat lines have been characterized using TRAP (target region amplification polymorphism) and SSR (simple sequence repeat) markers and seed storage proteins. This research resulted in development of about 800 chromosome-specific TRAP markers, identification of unique alleles for glutenin subunits and gliadins, and mapping of introgressed chromosome segments. The developed TRAP markers are currently being used for wheat genome mapping, chromosome identification, and genetic diversity studies. The glutenin subunits from chromosomes 1A, 1B, and 1D, and gliadins for 6A, 6B, and 6B, have been successfully used in the development of a new set of durum D-genome chromosome substitution lines and for transferring good bread-making quality from bread wheat to durum wheat cultivars. SSR markers closely flanking Tsn1, which confers sensitivity to a host selective toxin produced by the tan spot fungus, are being used to eliminate the sensitivity locus from commercial cultivars of bread wheat.