|Stockinger, Eric - OHIO STATE UNIVERSITY|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: January 14, 2005
Publication Date: January 15, 2005
Citation: Garvin, D.F., Stockinger, E.J. 2005. Development of a U.S. collection of inbred Brachypodium distachyon lines and the evaluation of genome microsynteny with cool season grass crops [abstract]. Plant and Animal Genome Abstracts. Paper No. 373. Technical Abstract: Brachypodium distachyon has been proposed as a potential model species for the cool season grasses, which includes many economically important crops such as wheat, barley, rye, and oats. However, many steps remain to be taken before Brachypodium can be exploited fully as a model species. We have developed a set of inbred lines of Brachypodium that can serve as reference genotypes for research and are using these genotypes to analyze both the structure and composition of the Brachypodium genome and the degree of synteny between Brachypodium and cool season grass crops. To date, a collection of inbred Brachypodium lines has been developed from 27 separate Brachypodium populations by single seed descent. Using flow cytometry, putative diploid genotypes have been identified from this collection and these results are being confirmed by chromosome counts. A bacteriophage lambda genomic library was constructed from a diploid Brachypodium line. This library was screened to identify clones encompassing the region homologous to the barley chromosome 5H C-Repeat Binding Factor locus, a region that spans approximately 100 kb in barley and is implicated in the control of freezing tolerance and winter survival in both barley and wheat. Comparative sequence analysis between this genome region in Brachypodium and barley shall provide an estimate of the level of microsynteny between Brachypodium and cool season grass crops. The annual habit of Brachypodium, coupled with the small genome size of diploid lines, confirms the potential of this species as a model for cool season grasses.