Submitted to: Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting
Publication Type: Other
Publication Acceptance Date: August 11, 2004
Publication Date: October 31, 2004
Citation: Halvorson, J.J., Gonzalez, J.M. 2004. Patterns of Recovery of Bradford Total Protein (Glomalin) from Appalachian Soils: Effects of Land Use, Depth and Extraction Reagent. IN ASA-CSSA-SSSA-CSSS Abstracts 2004 [CD-ROM]. ASA, CSSA, and SSSA, Madison, WI. Technical Abstract: Total protein, determined by the Bradford assay, is thought to represent glomalin, an important soil glycoprotein related to aggregation and soil carbon storage. We measured Bradford reactive protein (BRP) in soil from pastures, hayfields, cultivated fields or naturalareas in southwestern West Virginia. We found highest concentrations of BRP near the soil surface, decreasing significantly with depth. Soil from naturalareas contained highest concentrations of BRP compared to pastures, hayfields, or cultivated fields. Extracting soil with several different reagents indicated differences in BRP recovery efficiency possibly related to net electrical charge of the extractant and more apparent in cultivated soils than in naturalareas.