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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #169447
Title: CRYOPRESERVATION OF FRUIT AND SMALL FRUIT GERMPLASM BY VITRIFICATION

Author
item KUSHARENKO, S. - INST OF PLANT PHYSIOLOGY
item KOVALCHUK, I. - INST OF PLANT PHYSIOLOGY
item TURDIEV, T. - INST OF PLANT PHYSIOLOGY
item Reed, Barbara

Submitted to: Research Collection of Agricultural Academy, Almaty
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/20/2004
Publication Date: 7/20/2004
Citation: Kusharenko, S.V., Kovalchuk, I.Y., Turdiev, T.T., Reed, B.M. 2004. Cryopreservation of fruit and small fruit germplasm by vitrification. In: Proc. Intern. Sci. Conf. The Strategy of Scientific Ensuring of Horticulture: Reality and Perspectives. Almaty Agricultural University. p. 150-154. (in Russian).

Interpretive Summary: Cryopreservation is being developed for storing important fruit germplasm at the Institute of Plant Physiology, Genetics and Bioengineering, Almaty, Kazakhstan. Adaptation of two widely used cryopreservation techniques were found suitable for blackberry and black current storage in liquid nitrogen. Optimization of cold acclimation treatments greatly improved the recovery of many types of these berry crops. The modifications made will make it possible to keep plants stored for decades.

Technical Abstract: Cryopreservation is being developed for storing important fruit germplasm at the Institute of Plant Physiology, Genetics and Bioengineering, Almaty, Kazakhstan. Adaptation of two widely used cryopreservation techniques were found suitable for blackberry and black current storage in liquid nitrogen. Optimization of cold acclimation treatments greatly improved the recovery of many types of these berry crops. The modifications made will make it possible to keep a long-term storage collection of these plants as insurance against loss in the field. Rubus shoots were cryopreserved using cold-acclimated plantlets cryopreserved with either vitrification or encapsulation-dehydration techniques. Viability and recovery of meristems was noted weekly for 6 weeks. For two modifications of the PVS2 vitrification method the recovery of cryopreserved meristems varied from 18.4 to 51.7%. Higher viability and recovery was obtained with the encapsulation-dehydration technique (57.1 ' 70.6%). For optimization of cryopreservation protocol for black currant the effect of cold acclimation on regrowth of cryopreserved meristems by encapsulation-dehydration was studied. In vitro plantlets were cold acclimated for 1 to 6 weeks (22'C with 8 h light / -1'C, 16 h dark). Regrowth of the meristems dissected from non-cold acclimated plantlets following freezing was 29.7% and this improved only slightly with 1 week cold acclimation (31.6%). Two weeks cold acclimation increased the regrowth to 77.3%. Further increase of cold acclimation duration to 3-6 weeks resulted in little growth recovery following cryopreservation by encapsulation-dehydration.