ARS | Publication request: USE OF CPG OLIGODEOXYNUCLEOTIDES TO STIMULATE PORCINE NEUTROPHILS IN VITRO AND TO REDUCE SALMONELLA CHOLERAESUIS COLONIZATION IN VIVO

Submitted to: Keystone Symposia
Publication Type: Abstract Only
Publication Acceptance Date: September 28, 2004
Publication Date: January 8, 2005
Citation: Genovese, K.J., He, H., Lowry, V.K., Kogut, M.H. 2005. Use of CpG oligodeoxynucleotides to stimulate porcine neutrophils in vitro and to reduce Salmonella choleraesuis colonization in vivo [abstract]. Innate Immunity to Pathogens Keystone Symposium. p. 48.

Technical Abstract: Neutrophil oxidative burst and degranulation activities were measured using CpG ODN in vitro stimulation. Neutrophils isolated from pigs 6 weeks of age were incubated with treatments for 1 hour at 39 C. Neutrophils had increased degranulation activity when treated with CpG ODN when compared to the activity of cells treated with non CpG. Neutrophils exhibited an increase in oxidative burst activity using CpG ODN. CpG ODN treatment resulted in levels of activity comparable to those observed with PMA stimulation. Two of the swine CpG ODN (p2006 and pD19) were chosen for oral administration to swine for in vivo studies to observe the effects on S. choleraesuis (SC) infection in swine. Pigs were orally administered 1 mg of CpG ODN and subsequently challenged with SC. SC cfu in cecal contents in one CpG ODN group were reduced by two to three logs when compared to control cfu and other CpG ODN groups. In addition, reductions in SC positive rectal swabs, liver, spleen, jejunal, ileal, and colon colonization were observed.