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United States Department of Agriculture

Agricultural Research Service

Title: Cloning and Sequencing of the Bovine Fas-Associated Death Domain Protein (Fadd) Cdna

Authors
item Connor, Erin
item Szperka, Michael
item Bannerman, Douglas

Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: September 15, 2004
Publication Date: September 15, 2004
Citation: Connor, E.E., Szperka, M.E., Bannerman, D.D. 2004. Cloning and sequencing of the bovine fas-associated death domain protein (fadd) cdna. Germplasm Release. Accession Number AY725483.

Interpretive Summary: This is a Germplasm Release. No interpretive Summary required.

Technical Abstract: Fas-associated death domain protein (FADD) is an adapter molecule expressed in many tissues that mediates cell apoptotic signaling. The full-length sequence of the bovine FADD cDNA was determined by reverse-transcription PCR using mRNA isolated from cultured bovine aortic endothelial cells and cloning of the resulting product into a plasmid vector for DNA sequencing. Sequencing of bovine FADD cDNA revealed approximately 83 and 80% nucleotide sequence identity to its human and murine homologues, respectively. A bovine FADD dominant-negative construct was developed to examine bacterial lipopolysaccharide (LPS)- and TNF-a-induced apoptosis in bovine endothelial cells. GenBank Accession No. AY725483.

Last Modified: 4/19/2014
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