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United States Department of Agriculture

Agricultural Research Service

Title: On the Hunt for New Molecular Markers

Authors
item Scheffler, Jodi
item Scheffler, Brian
item Ray, Jeffery
item Taliercio, Earl

Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: October 10, 2004
Publication Date: N/A

Technical Abstract: Molecular based markers have been developed for a number of plant species. These markers have subsequently been used for a variety of purposes including diagnostic markers for resistance genes, creating "fingerprints" for identification, and assembling genomic maps. Most of the major crop species have well established maps that combine a number of molecular and physical markers that are freely available for use by the research community. Initially cotton lagged behind in developing an easily acdessible map; however, recent coordinated efforts by cotton researchers have accelerated the process. Cotton scientists can now take advantage of the work already done in other crops to increase the speed and efficiency of these efforts. To date, Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP) and Simple Sequence Repeat (SSR) markers have been used successfully in cotton. SSR markers can be derived from several sources including random genomic fragments and Expressed Sequence Tags (ESTs). Useful markers can be found both within and outside the genus Gossypium. Preliminary results indicate that soybean (Glycine max) and Arabidopsis thaliana SSRs can be informative markers for cotton.

Last Modified: 11/23/2014