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United States Department of Agriculture

Agricultural Research Service

Title: Mosaic (Msc) Cucumbers Regenerated from Independent Cell Cultures Possess Different Mitochondrial Rearrangements

Authors
item Bartoszewski, G - WARSAW AGRL UNIV-POLAND
item Malepszy, S - WARSAW AGRL UNIV-POLAND
item Havey, Michael

Submitted to: Current Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 27, 2004
Publication Date: August 15, 2004
Citation: Bartoszewski, G., Malepszy, S., Havey, M.J. 2004. Mosaic (MSC) cucumbers regenerated from independent cell cultures possess different mitochondrial rearrangements. Current Genetics. 45:45053.

Interpretive Summary: Cucumber plants regenerated from cell cultures occasionally produce progenies showing a strong mosaic (MSC) phenotype on cotyledons and leaves. The MSC phenotype shows paternal transmission and the genetic bases of independently derived MSC lines are unknown. Because cucumber mitochondrial genome is paternally transmitted we evaluated independently derived MSC cucumber lines for differences in the mitochondrial DNA. DNA polymorphisms were revealed near the rrn5/rrn18, nad5-exon2, rpl5 coding and JLV5 non-coding regions. Polymorphisms revealed by rrn18 and nad5-exon2 were due to one rearrangement bringing together these two regions. The polymorphism revealed by rpl5 gene was unique to MSC16 and was a rearrangement placing the rpl5 region next to the forward junction of the JLV5 deletion. Five different hybridization patterns were revealed between 17 independently derived tissue culture lines. This research will be of interest to plant geneticists and breeders because passage of cucumber through cell culture may be a unique method to produce rearrangements affecting mitochondrial gene expression.

Technical Abstract: Cucumber plants regenerated from somatic cells occasionally produce progenies with a strong mosaic (MSC) phenotype on cotyledons and leaves. The MSC phenotype shows paternal transmission and the genetic bases of independently derived MSC lines are unknown. Because cucumber mitochondrial genome is paternally transmitted we hybridized 15 cosmids covering the mitochondrial genome of Arabidopsis thaliana to DNA blots of independently derived MSC lines from tissue cultures and wild-type lines. Polymorphism was revealed near the rrn5/rrn18, nad5-exon2, rpl5 coding and JLV5 non-coding regions. Polymorphisms revealed by rrn18 and nad5-exon2 were due to one rearrangement bringing together these two regions. The polymorphism revealed by rpl5 gene was unique to MSC16 and was a rearrangement placing the rpl5 region next to the forward junction of the JLV5 deletion. Five different hybridization patterns were revealed between 17 independently derived tissue culture lines. Passage of cucumber through cell culture may be a unique method to produce novel mitochondrial DNA rearrangements affecting mitochondrial gene expression.

Last Modified: 12/25/2014
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