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United States Department of Agriculture

Agricultural Research Service

Title: Sweetpotato Whitefly Honeydew Production and Nymph Development When Feeding on Different Host Plants in the Laboratory, 2002

Authors
item Henneberry, Thomas
item Jech, Lynn
item De La Torre, Theresa
item Maurer, Jamie

Submitted to: Arthropod Management Tests
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 1, 2003
Publication Date: October 6, 2003
Citation: Henneberry, T.J., Jech, L.J., De La Torre, T.M., Maurer, J.C. 2003. Sweetpotato whitefly honeydew production and nymph development when feeding on different host plants in the laboratory, 2002. Arthropod Management Tests M7.

Interpretive Summary: Two leaf-clip cages with removable plastic bottoms were installed on each of two plants of each species. Five Sweetpotato whitefly (SPW) adult females were placed in each clip cage on each leaf Honeydew was collected for 24 h on the clip cage bottoms. Cages were removed and adults and eggs laid on leaves counted. Cage bottoms with honeydew were frozen until lyophilized and processed for High Performance Liquid Chromatography (HPLC) analysis. Eggs were checked daily for hatch. When first instar nymphs (crawlers) settled on leaves for feeding, five to seven were encircled with an indelible pencil. Each nymph was checked daily for stage of development. When late- third, early- fourth instars ("red-eye nymphs") occurred, leaf-clip cages were positioned to encircle living nymphs. Honeydew was collected for 24 h, cages removed, nymphs counted, and removable cage bottoms with honeydew were held in a freezer as described.Honeydew was washed ITom all leaf-cage bottoms with 3 ml of warm deionized water and ITozen in 15 ml plastic test tubes. Frozen honeydew samples were lyophilized and reconstituted in 125 of deionized water. Glucose, fructose trehalulose and sucrose in the samples were determined using HPLC and sampled sugars quantified by comparison with peak areas of known sugar standards. The experiments were conducted in RCB designs with four to eight replications on greenhouse benches. All data were analyzed using ANOVAs and LSD mean separations after a significant F test.

Technical Abstract: Two leaf-clip cages with removable plastic bottoms were installed on each of two plants of each species. Five Sweetpotato whitefly (SPW) adult females were placed in each clip cage on each leaf Honeydew was collected for 24 h on the clip cage bottoms. Cages were removed and adults and eggs laid on leaves counted. Cage bottoms with honeydew were frozen until lyophilized and processed for High Performance Liquid Chromatography (HPLC) analysis. Eggs were checked daily for hatch. When first instar nymphs (crawlers) settled on leaves for feeding, five to seven were encircled with an indelible pencil. Each nymph was checked daily for stage of development. When late- third, early- fourth instars ("red-eye nymphs") occurred, leaf-clip cages were positioned to encircle living nymphs. Honeydew was collected for 24 h, cages removed, nymphs counted, and removable cage bottoms with honeydew were held in a freezer as described.Honeydew was washed from all leaf-cage bottoms with 3 ml of warm deionized water and frozen in 15 ml plastic test tubes. Frozen honeydew samples were lyophilized and reconstituted in 125 of deionized water. Glucose, fructose trehalulose and sucrose in the samples were determined using HPLC and sampled sugars quantified by comparison with peak areas of known sugar standards. The experiments were conducted in RCB designs with four to eight replications on greenhouse benches. All data were analyzed using ANOVAs and LSD mean separations after a significant F test.

Last Modified: 9/2/2014
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